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Ornithine decar☐ylase assay using ion-exchange paper

✍ Scribed by Jeffrey L. Clark

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 470 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90213-x

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✦ Synopsis

A new, quantitative radioassay for omithine decarboxylase is described which involves determination of putrescine, the organic product of the reaction. The assay is based on the selective adsorption and retention of putrescine by Whatman CM 82 paper, a weak cation exchanger, and is compatible with the variety of assay conditions routinely employed in which released r4C0, is measured. A rapid, semiquantitative variation of the assay which should be useful for mass screening procedures is also presented.

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