A novel mass spectrometry-based methodology using electrospray ionization (ESI) is described for the detection of protein-protein [interferon (IFN)-γ dimer] and protein-ligand [ras-guanosine diphosphate (GDP)] noncovalent interactions. The method utilizes ESI from aqueous solution at appropriate pH.
Oriented immobilisation of histidine-tagged protein and its application in exploring interactions between ligands and proteins
✍ Scribed by Zhao, Xinfeng; Li, Qian; Xiao, Chaoni; Zhang, Yajun; Bian, Liujiao; Zheng, Jianbin; Zheng, Xiaohui; Li, Zijian; Zhang, Youyi; Fan, Taiping
- Book ID
- 125341962
- Publisher
- Springer
- Year
- 2014
- Tongue
- English
- Weight
- 508 KB
- Volume
- 406
- Category
- Article
- ISSN
- 1618-2650
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