Orientation-Selective Incorporation of Transmembrane F0F1 ATP Synthase Complex from Micrococcus luteus in Polymer-Supported Membranes
✍ Scribed by Murat Tutus; Fernanda F. Rossetti; Emanuel Schneck; Giovanna Fragneto; Friedrich Förster; Ralf Richter; Thomas Nawroth; Motomu Tanaka
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 847 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1616-5187
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
We report the vectorial incorporation of a highly asymmetric F~0~F~1~ ATP synthase complex from Micrococcus luteus into polymer‐supported membranes. Dynamic light scattering and cryo electron microscopy confirm that the use of weak surfactants (bile acid) allows for the non‐disruptive protein incorporation into lipid vesicles. Spreading of vesicles with ATP synthase onto a cellulose support results in a homogeneous distribution of proteins, in contrast to a patchy image observed on bare glass slides. The orientation of ATP synthase can be identified using an antibody to the ATP binding site as well as from topographic profiles of the surface. The method to “align” transmembrane proteins in supported membranes would open a possibility to quantify protein functions in biomimetic model systems.
magnified image