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Orientation of “plus” genes at the mating-type locus in homothallic fission yeast

✍ Scribed by Egel, Richard

Publisher
Springer
Year
1978
Tongue
English
Weight
461 KB
Volume
161
Category
Article
ISSN
0026-8925

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✦ Synopsis

Results of four crosses are presented which indicate that, in Schizosaccharomyces pombe the plus segment of the mating-typing locus is regulated from a central position, in between of the plus and the minus segment. This conclusion is based on the mapping of a plus-restraining entity r, which is revealed by recombination in certain crosses, or by mutation to unstabe negative strains. To this end, a meiosis-defective mating-type mutation was used as a marker for the plus segment, by taking advantage of its suppressibility by a nonsense suppressor. This suppressibility was substanitated by an additional cross.

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