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Orientation effects in DNA during gel-electrophoresis

✍ Scribed by A.N. Semenov; A.V. Subbotin


Book ID
116049652
Publisher
Elsevier Science
Year
1990
Weight
378 KB
Volume
32
Category
Article
ISSN
0032-3950

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DNA orientation during gel electrophores
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Intensities of polarized fluorescence from ethidium bound to phage h DNA undergoing agarose gel electrophoresis were measured. The intensities were strongly field dependent at voltage gradients of 8 V/cm, consistent with a partial orientation of DNA helices in the direction of electrophoresis about