Organotin compounds as energy-potentiated uncouplers of rat liver mitochondria
✍ Scribed by Ian F Connerton; David E Griffiths
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 581 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0268-2605
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✦ Synopsis
and the accompanying pH gradient (ApH) in estimates of protonmotive force (Ap) in isolated rat liver mitochondria in an apparent concentrationdependent manner and in minimal halide media (5 pmol dm-3). Under these conditions the concentrations of organotin required to produce a drop of 80-120 mV in Ap approach or are in excess of those required to inhibit ATP-synthase activity, which are at least three-fold greater than those which inhibit ATP hydrolytic activity. The addition of exogenous chloride ion did not appreciably alter the steady-state or rate estimates of triorganotinmediated membrane potential (A$) depolarization.
The evidence indicates that triorganotins possess an uncoupling effect which is independent of halide/ hydroxyl exchange or direct inhibition of the ATPase complex. The activity of various triorganotins may be best understood according to their abilities to uncouple or directly inhibit oxidative phosphorylation at the enzymic level, in the absence of halide/hydroxyl exchange.