Organogenesis and tuberization in cultures ofDioscorea floribunda

✍ Scribed by Jayanti Sengupta; G. C. Mitra; A. K. Sharma

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 630 KB
• Volume: 3
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00043084

✦ Synopsis

Callus cultures were established from node and internode segments of Dioscorea floribunda Mart. & Gal. Both Murashige and Skoog's and modified White's medium supported callusing as well as organogenesis when supplemented with either 2,4-D or NAA in combination with BAP or Kn. On development of shoot primordia, calli were transferred to unsupplemented, half strength MS basal medium. This procedure led to the increase in formation of shoots. Several 'crops' of shoots were obtained from single differentiating callus cultures by excising the shoots and subculturing the residual part. Seventy percent of plantlets survived rooting and transfer to soil.

When they were maintained in half-strength MS basal medium and 0.5 mg 1-1 of NAA, 70% of plantlets formed aerial tubers at nodes. These tubers produced both roots and shoots and could be detached from the mother plant.