✦ LIBER ✦

Organization of the ribosomal ribonucleic acid genes in various wild-type strains and wild-collected strains of Neurospora

✍ Scribed by Russell, Peter J. ;Wagner, Sheryl ;Rodland, Karin D. ;Feinbaum, Rhonda L. ;Russell, Jennifer P. ;Bret-Harte, Marion S. ;Free, Stephen J. ;Metzenberg, Robert L.

Publisher: Springer
Year: 1984
Tongue: English
Weight: 821 KB
Volume: 196
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00328060

No coin nor oath required. For personal study only.

✦ Synopsis

The organization of the ribosomal DNA (rDNA) repeat unit in the standard wild-type strain of Neurospora crassa, 74-OR23-1A, and in 30 other wild-type strains and wild-collected strains of N. crassa, . tetrasperma, N. sitophila, N. intermedia, and N. discreta isolated from nature, was investigated by restriction enzyme digestion of genomic DNA, and probing of the Southern-blotted DNA fragments with specific cloned pieces of the rDNA unit from 74-OR23-1A. The size of the rDNA unit in 74-OR23-1A was shown to be 9.20 kilobase pairs (kb) from blotting data, and the average for all strains was 9.11 + 0.21 kb; standard error = 0.038; coefficient of variation (C.V.) = 2.34%. These data indicate that the rDNA repeat unit size has been highly conserved among the Neurospora strains investigated. However, while all strains have a conserved HindIII site near the 5' end of the 25 S rDNA coding sequence, a polymorphism in the number and/or position of HindIII sites in the nontranscribed spacer region was found between strains. The 74-OR23-1A strain has two HindIII sites in the spacer, while others have from 0 to at least 3. This restriction site polymorphism is strain-specific and not species-specific. It was confirmed for some strains by restriction analysis of clones containing most of the rDNA repeat unit. The current restriction map of the 74-OR23-1A rDNA repeat unit is presented.

📜 SIMILAR VOLUMES

Xanthommatin biosynthesis in wild-type a

Xanthommatin biosynthesis in wild-type and mutant strains of the Australian sheep blowflyLucilia cuprina

✍ K. M. Summers; A. J. Howells 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 458 KB

The synthesis of eye pigments has been studied in the seven eye color mutants of the Australian sheep blowfly, Lucilia cuprina. Six appears to be affected primarily in the synthesis of xanthommatin. In wild type, the onset of xanthommatin biosynthesis occurs midway through metamorphosis. Development

Regulation of the nitrate-reducing syste

Regulation of the nitrate-reducing system enzymes in wild-type and mutant strains of Chlamydomonas reinhardii

✍ Fernández, Emilio ;Cárdenas, Jacobo 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 645 KB

Reductive biotransformation by wild type

Reductive biotransformation by wild type and mutant strains of Saccharomyces cerevisiae in aqueous-organic solvent biphasic systems

✍ P. Nikolova; O. P. Ward 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 769 KB

Light-induced Dephosphorylation of a 33

Light-induced Dephosphorylation of a 33 kDa protein in the wild-type strain of Neurospora crassa: The regulatory mutants wc-i and wc-2 are abnormal

✍ Frank-R. Lauter; Vincenzo E.A. Russo 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 625 KB

Light induces the dephosphorylation of a 33 kdalton protein within 8 min in the wild-type strain of Neurospora crassa. The regulatory mutants, wc-1 and wc-2, have an altered pattern of phosphoproteins in darkness and also after irradiation. Because the wc genes have previously been implicated in pho

Ribosomal genes of Neurospora crassa: Co

Ribosomal genes of Neurospora crassa: Constancy of gene number in the conidial and mycelial phases, and homogeneity in length and restriction enzyme cleavage sites within strains

✍ Rodland, Karin D. ;Russell, Peter J. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 274 KB

We report the results of experiments which, while not specifically designed to study the possibility of rDNA amplification during different developmental stages in the N. crassa life cycle, clearly indicate a relative constancy in the rDNA content of conidia (asexual spores) and mycelial cells. We a

Studies on the female sterile mutant rud

Studies on the female sterile mutant rudimentary ofDrosophila melanogaster. II. An analysis of aspartate transcarbamylase and dihydroorotase activities in wild-type and rudimentary strains

✍ Anne Fausto-Sterling 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 738 KB

The activities of the enzymes aspartate transcarbamylase (ATCase) and dihydroorotase (DHOase) were determined in adult females from a wild-type strain and from eight different alleles of the X-linked mutation rudimentary (r) of Drosophila melanogaster. The alleles chosen span the genetic map of the