Organization and regulation of the dynein microtubule motor

✍ Scribed by Stephen M. King

• Publisher: Elsevier Science
• Year: 2003
• Tongue: English
• Weight: 231 KB
• Volume: 27
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1016/s1065-6995(02)00337-2

✦ Synopsis

The outer dynein arm from flagella of the green alga Chlamydomonas reinhardtii is one of the bestcharacterized members of this class of microtubulebased molecular motor. This dynein consists of 13 different components and has a total mass of 2MDa (Pfister et al., 1982). Genetic analysis of mutants defective for various dynein components has revealed that nearly all these proteins are essential for assembly of the intact dynein particle (Table 1). The complex is built around three heavy chains (HCs, the , , ; w510kDa each) that exhibit different enzymatic and motor properties (Fig. 1a ). Each HC contributes an N-terminal stem domain that interacts with other HCs and an additional subcomplex of conserved intermediate and light chains (ICs and LCs). This IC/LC complex is required for attachment of this dynein to its target location within the flagellar axoneme, which is marked