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Organization and expression of hepatitis B sequences cloned from hepatocellular carcinoma tissue DNA

✍ Scribed by Anne Dejean; Guido Carloni; Christian Bréchot; Pierre Tiollais; Simon Wain-Hobson

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
542 KB
Volume
20
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

We have constructed a phage λ library of liver DNA fragments from West African patient who died of liver failure due to advanced hepatocellular carcinoma. Four hepatitis B virus (HBV) DNA‐carrying recombinants have been isolated, one clone (λÍ22) being analyzed in greatest detail. It contains approximately 3.8 kb of HBV DNA without detectable deletions or rearrangements. One site of integration lies close to the nick in free viral DNA. The restriction map of the HBV sequences is close to those published for the ay subtype. Coconvection of mouse Ltk^−^ cells with λÍ22 and cloned: thymidine kinase gene results in the expression of gene S and the excretion of hepatitis B surface antigen (HBsAg) particles into the culture supernatant.

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