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Organic alkali-labile phosphates in biological materials: A generic assay to detect vitellogenin in biological tissues

✍ Scribed by F. Gagné; C. Blaise

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
73 KB
Volume
15
Category
Article
ISSN
1520-4081

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✦ Synopsis

A simple method is described for the determination of the levels of phosphorylated lipopro-Ž . teins e.g., vitellins and vitellogenins in biolog-Ž . ical fluids or tissues. Vitellogenin Vg is a very high density lipophosphoprotein and its synthesis is activated by estrogens in oviparous organisms.

2.0 SCOPE

This method is usually applicable to any biological fluids or tissues rich in Vg. This assay was used on the following type of samples:

Ž . i Cell culture media containing secreted Vg from fish hepatocytes.

Ž .

ii Blood plasma from fish or hemolymph from mollusks.

📜 SIMILAR VOLUMES

Acute and Chronic Toxicity of Veratrum V
Acute and Chronic Toxicity of Veratrum Viride††The Veratrum viride studied was the whole-powdered drug marketed by Irwin, Neisler & Co. under the name, Vertavis. The work reported herein was supported by a grant from this firm. The batch of powder used in these experiments was stated by the manufacturer to have been assayed biologically by the Craw method on Daphnia magna. One Craw unit of the material studied was stated to be 15.6mg. With regard to this assay, Mr. R. O. Craw has been so kind as to allow us to publish a brief explanation: A reference lot of Yerolrum oirid; powder was set-aside as a standard and a 10% w/v aqueous extract was made of this powder. The average median length of life in 50 assays of 1000 daphnia on immersion in the solution was determined to be 2.22 hours. The daphnia were considered dead when the heart was found to be in systolic standstill on microscopic examination. Succeeding lots of powder were assayed and their potency determined by relating the median length of life of daphnia to 2.22 hours, which is one unit. Twenty assays of 10 animals each were used on each lot of powder.‡‡The authors wish to thank Dr. J. F. A.,McManus of the Department of Pathology for a pathologic report on the tissues, and Mr. James Thomas and Mrs. Bobby E. Teague for technical assistance.
✍ Perdue, Adoline S. ;Bell, Frances ;Starnes, W.R. ;Hitchcock, Philip ;Teague, R.S 📂 Article 📅 1950 🏛 Elsevier ⚖ 346 KB 👁 1 views