Organ culture of rat white adipose tissue
✍ Scribed by Jiří Frohlich; Alan Vost; Charles H. Hollenberg
- Book ID
- 115736529
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 681 KB
- Volume
- 280
- Category
- Article
- ISSN
- 0005-2760
No coin nor oath required. For personal study only.
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## Abstract In order to compare the morphologic response of white and brown fat to various hormones __in vitro__ the technique of organ culture was used in this study. Explants of white and brown fat from young mice fasted for 48 hours were exposed to culture media supplemented with insulin, thyrox
Fragments of human adipose tissue were maintained in culture for 1 week in a medium containing I mU/ml insulin and 100 ng/ml dexamethasone. Under these conditions lipoprotein lipase activity was present in human adipose tissue fragments which converted [~¢C]glucose to t4CO2 and [14C]triglyceride. Bo