Ordering of six polymorphic dna markers important in the delineation of 3p deletions in neoplasia

✍ Scribed by Amanda C. Heppell-Parton; Pamela H. Rabbitts; Donna G. Albertson

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 646 KB
• Volume: 4
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870040307

✦ Synopsis

Using fluorescence in situ hybridisation (FISH) the chromosomal location and relative order of six human chromosome 3 probes has been determined. The sensitivity of the technique has enabled the relative mapping of probes carrying inserts as small as 500 basepairs (bp), thus allowing the following proximal-distal probe order to be proposed: D3S30 (3p I3-14). D3S4 (3p 13-14), D3S2 (distal 3p 14), D3S32 (3p2 I), D3S48E (3p2 I -23), and D3S I I (3~22-23). These data combined with the deletion mapping data of other researchers raise the possibility that the loss of more than one region of the short arm of chromosome 3 may be important in the development of small cell lung cancer. Genes Chrorn Cancer 4:228-234 (1992).