Optosensing of d-glucose with an immobilized glucose oxidase minireactor and an oxygen room-temperature phosphorescence transducer
✍ Scribed by M.J. Valencia-González; Y.M. Liu; M.E. Díaz-García; A. Sanz-Medel
- Book ID
- 102981069
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 682 KB
- Volume
- 283
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Glucose oxidase was immobilized covalently on a nylon membrane and the oxygen consumption was followed via the changes in the room-temperature phosphorescence. (RTP) of an oxygen-sensitive metal chelate whose RTP is quenched dynamically by molecular oxygen. The metal chelate was immobilized on an anion-exchange resin and packed into a conventional flow-through cell. As result of the glucose oxidation in the presence of the enxyme, a certain amount of oxygen was consumed, which in turn was indicated by the RTP of the chelate. Measurements were made in flowing air-saturated solutions containing 0.1 M acetate buffer (PH 5.9). The system is linear for 0.5-2.5 mM glucose with a relative standard deviation of 1.6% at 2 mM (ten measurements). The detection limit is 8 x lo-' M and the application of the system to glucose determhration in serum and beverages is demonstrated.