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Options for biochemical production of 4-hydroxybutyrate and its lactone as a substitute for petrochemical production

✍ Scribed by C. Efe; Adrie J.J. Straathof; Luuk A.M. van der Wielen

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
231 KB
Volume
99
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

Options are discussed for biochemical production of 4‐hydroxybutyrate (4‐HB) and its lactone, gamma‐butyrolactone (GBL), from renewable sources. In the first part of the study, the thermodynamic feasibility of four potential metabolic pathways from glucose to 4‐HB are analyzed. The calculations reveal that when the pathways are NAD^+^ dependent the intermediate succinate semialdehyde (SSA) accumulates leading to low 4‐HB yields at equilibrium. For NADP^+^ dependent pathways the calculated yield of 4‐HB improves, up to almost 100%. In the second part of this study, continuous removal of 4‐HB from the solution is considered to shift SSA conversion into 4‐HB so that SSA accumulation is minimized. One option is the enzymatic production of GBL from 4‐HB. Candida antarctica Lipase B shows good lactonization rates at pH 4, but unfortunately this conversion cannot be performed in‐vivo during 4‐HB production because of the neutral intracellular pH. Biotechnol. Bioeng. 2008;99: 1392–1406. © 2007 Wiley Periodicals, Inc.

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