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Optimum conditions for the radioimmunoprecipitation assay for the major internal protein of bovine leukemia virus

✍ Scribed by Mary Jo F. Schmerr; Kathryn R. Goodwin

Book ID
119098715
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
293 KB
Volume
2
Category
Article
ISSN
0165-2427

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## Abstract The initiation of translation in hepatitis C virus (HCV) occurs at the internal ribosome entry site (IRES) located at the 5′‐end of its genomic RNA. To study the function of HCV IRES, we constructed a reporter plasmid that generates a bicistronic mRNA encoding two fluorescent proteins: