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Optimizing19F NMR protein spectroscopy by fractional biosynthetic labeling

✍ Scribed by Julianne L. Kitevski-LeBlanc; Ferenc Evanics; R. Scott Prosser


Book ID
106401735
Publisher
Springer Netherlands
Year
2010
Tongue
English
Weight
513 KB
Volume
48
Category
Article
ISSN
0925-2738

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Biosynthetically directed fractional 13 C labeling of the proteinogenic amino acids is achieved by feeding a mixture of uniformly 13 C-labeled and unlabeled carbon source compounds into a bioreaction network. Analysis of the resulting labeling pattern enables both a comprehensive characterization of