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Optimizing of heterologous expression of human recombinant His-tagged prethrombin-2 in Escherichia coli

✍ Scribed by Krahulec, Jan; Osadska, Michaela; Slikova, Eva; Halaszova, Hana; Stuchlik, Stanislav; Turna, Jan

Book ID: 123311140
Publisher: Elsevier Science
Year: 2013
Tongue: English
Weight: 64 KB
Volume: 24
Category: Article
ISSN: 0958-1669
DOI: 10.1016/j.copbio.2013.05.357

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## Abstract An expression system based on __Escherichia coli__ and the T5 promoter allowed the overproduction of a his‐tagged rhamnulose‐1‐phosphate aldolase (RhuA; EC 4.1.2.19), an enzyme with applications in the production of deoxyazasugars and deoxysugars compounds. Shake flask and bioreactor cu