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Optimizing expression and engineering the properties of a clostridial ferredoxin.

✍ Scribed by V. Davasse; I. Quinkal; J. Gaillard; J.-M. Moulis


Book ID
103569582
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
88 KB
Volume
51
Category
Article
ISSN
0162-0134

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## Abstract The model iron‐sulfur (Fe‐S) protein ferredoxin (Fd) from __Synechocystis sp__. PCC 6803 has been simultaneously produced and matured in a cell‐free production system. After 6 h of incubation at 37Β°C, Fd accumulated to >450 Β΅g/mL. Essentially all was soluble, and 85% was active. Product