Optimizing copper-bicinchoninate carbohydrate detection for use with water-elution high-performance liquid chromatography: A technique to measure the major mono- and oligosaccharides in small pieces of wheat endosperm
✍ Scribed by T.David Ugalde; J.Pieter M. Faber; Colin F. Jenner
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 527 KB
- Volume
- 449
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
Modifications are described to the copper-bicinchoninate detection of reducing sugars to increase sensitivity when used in conjunction with water-elution highperformance liquid chromatography and post-column catalytic hydrolysis of some oligosaccharides to a reducing form. The lowest limit of detection, taken to be the amount of substance that produces a peak height twice the noise level, was about 1 ng for a number of reducing and non-reducing sugars. Colour formation was linear (< 5% deviation) and reproducible (S.D. < 10% at extremes) for detection response equivalent to between 10 ng and 2.5 pg glucose. Use of this technique to measure the major monosaccharides and oligosaccharides in very small pieces of wheat endosperm is described.