Optimized isocratic conditions for the simultaneous determination of serotonin precursors and metabolites by reversed-phase high-pressure liquid chromatography with electrochemical detection

✍ Scribed by Andrew J. Falkowski; Robert Wei

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 527 KB
• Volume: 115
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90011-7

✦ Synopsis

We describe an analytical procedure for the simultaneous determination of S-hydroxyindole derivatives using high-pressure liquid chromatography with electrochemical detection. The procedure clearly resolves Shydroxytryptophan, 5-hydroxytryptamine, Shydroxytryptophol, and S-hydroxyindole-3-acetic acid. The C-l 8 extraction column methodology and high-pressure liquid chromatography-electrochemical detection parameters have been developed to provide a rapid, sensitive, and reproducible quantitative determination of these S-hydroxyindoles with picogram sensitivity. Chromatograms obtained from the analysis of whole normal mouse brain by the present technique clearly resolve the S-hydroxyindoles and appear to be uncomplicated by interfering substances.