Optimized enzymatic synthesis of geranyl butyrate with lipase AY from candida rugosa
✍ Scribed by Chwen-Jen Shieh; Casimir C. Akoh; Lisa N. Yee
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 393 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
Response surface methodology (RSM) and five-level, five-variable central composite rotatable design (CCRD)
were used to evaluate the effects of synthetic variables, such as reaction time (1-9 h), temperature (25-65"C), enzyme amount (10-50%), substrate molar ratio of geraniol to tributyrin (1 :0.33-1 : I), and added water amount (0-20%) on molar percent yield of geranyl butyrate, using lipase AY from Candida rugosa. Reaction time and temperature were the most important variables and substrate molar ratio had no effect on percent molar conversion. Based on contour plots, optimum conditions were: reaction time 9 h, temperature 35"C, enzyme amount 50%, substrate molar ratio 1 :0.33, and added water 10%. The predicted value was 100% and actual experimental value was 96.8% molar conversion.