Optimization studies of components in enzymatic cholesterol reagents containing cholesterol oxidase from Nocardia erythropolis,Streptomyces sp, or Pseudomonas fluorescens
✍ Scribed by Porntip H. Lolekha; Yaovaluk Teerajetkul
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 748 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
Although enzymatic methods for serum mUL, and phosphate buffer, pH 7.0. Lower cholesterol determination are widely used reaction sensitivity and lower cholesterol linin clinical laboratories! little is known about earity, < 18.1 mmol/L (700 mg/dL), could be the optimization of each component in en-obtained by using lower components than zymatic reagents. We investigated the op-those suggested above. Pseudomonas timal components in the reagents fluorescens were an improper source for containing cholesterol oxidase isolated cholesterol oxidase; either Nocardia from Nocardia erythropolis, Streptomyces erythropolis or Streptomyces was suitable sp, or Pseudomonas fluorescens. The opti-cholesterol oxidase. We prefer using Strep-ma1 components in the reagents are: chofomyces sp cholesterol oxidase because of lesterol oxidase 250 (Nocardia erythropolis), its economical cost and longest reagent sta-250 (Sfreptomyces sp), or 300 (Pseudomo-bility. Sodium cholate must be included in nas fluorescens) UIL, cholesterol esterase the enzymatic reagent to prevent turbid-200 U/L, peroxidase 10,000 U/L, sodium ity. However, sodium cholate of > 5 mmoll cholate 3 mmol/L, 4-aminoantipyrine 0.5 L will suppress the reaction resulting in low mmOl/L, phenol 20 mmol/L, Triton X-100 2 cholesterol linearity. 01996 Wdey-Liss, Inc.