๐”– Bobbio Scriptorium
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Optimization of the release of undegraded material extracted from cells by ultrasound

โœ Scribed by W. T. Coakley; R. C. Brown; C. J. James; R. K. Gould

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
611 KB
Volume
16
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

Abstract

The time course of release of intact labile material from cells by cavitating ultrasound is examined. Curves are presented which show the yield of intact subcellular components obtainable from cells as a function of sonication time for various values of the ratio of inactivation rate to release rate. The general cases where inactivation of released product is concentration dependent (chemical) and concentration independent (mechanical) are considered.

For a flow system the time of attainment of equilibrium concentration of active product is analyzed as a function of flow rate, release rate, inactivation rate, and volume of chamber. Curves of optimal yields to be expected for batch and flow systems are presented. It is shown that sonochemical inactivation can be made negligible by sonication of high cell concentration suspensions.

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