Optimization of the photoionization detector for use with capillary columns

✍ Scribed by Jaramillo, L. F. ;Driscoll, J. N.

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 288 KB
• Volume: 2
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240020813

✦ Synopsis

Table 1 k-Values of 3-Ethinylcholestan-3a-oles Table 2 k-Values of 3-Ethinylcholestan-3p-oles Mobile Phase') Mobile Phase') R2) I I1 111 Rf 0.30 0.33 0.41 k 2.33 2.03 1.44 Rf 0.57 0.61 0.72 k 0.75 0.64 0.39 H C6H5 0.52 0.61 0.74 0.92 0.64 0.35 R2) I I1 111 Rf 0.12 0.14 0.20 k 7.33 6.1 4 4.00 Rf 0.18 0.21 0.28 k 4.56 3.76 2.57 Rf 0.1 7 0.21 0.30 (CHd5CH3 k 4.88 3.76 2.33 H C6H5 1) Mobile Phase I: benzene, 11: benzene + 0.1% (v/v) abs. ethanol, 111: Benzene + 0.5% (vlv) abs. ethanol.

The substituent R corresponds to the substituent R in Figures 1 and2.

2)

with the simple chromatographic system which was used, a valid relation between structure and k-value in a given pair of ethinylcholestanoles may be drawn.

Addition of ethanol (0.1 % (vlv) and 0.5% (vlv)) to the mobile phase has no drastic effect on the characteristic differences in the chromatographic behaviour of an epimeric pair of ethinylcholestanoles.