Optimization of the ion-exchange analysis of organic acids from fermentation

✍ Scribed by M.A. Eiteman; M.J. Chastain

• Book ID: 104104878
• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 593 KB
• Volume: 338
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(96)00426-6

✦ Synopsis

A method using high performance liquid chromatography (HPLC) is described to quantify 13 organic acids commonly found as products of fermentation. The isocratic method uses a 30cm sulfonated polystyrene divinyl benzene column with ultraviolet detection at 210nm to quantify acetic, butyric, citric, formic, fumaric, isobutyric, a-ketoglutaric, lactic, malic, propionic, pyruvic, succinic, and uric acids. Since all 13 acids are rarely found together in a single sample from a fermentation medium, a method is described to select the optimal temperature and eluent concentration for a given subset of these organic acids. This simple chromatographic optimization is applied to select conditions for the quantification of fermentation products of two anaerobic microorganisms, Fibrobacter succinogenes and Clostridium coccoides.