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Optimization of the expression of a foreign gene from galactose-inducible promoter in a recombinant yeast

✍ Scribed by Kong Hua Lin; Shinji Iijima; Shih-yow Huang; Fumio Hishinuma; Takeshi Kobayashi


Book ID
113396569
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
669 KB
Volume
72
Category
Article
ISSN
0922-338X

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## Abstract We report a new procedure to express recombinant human activin A using the methanolic yeast, __Pichia pastoris__. Optimization of culture procedures has involved comprehensive examination of the effects of culture vessel shape, volume of broth in the induction and expression cultures, m