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Optimization of the conditions for the expression of an anti-transthyretin scFv in Pichia pastoris

✍ Scribed by Symon Riedstra; Liliana Cerejo; Susana Brás; Paulo M.P. Costa; João Paulo M. Ferreira

Book ID
118472468
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
84 KB
Volume
131
Category
Article
ISSN
0168-1656

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## Abstract We report a new procedure to express recombinant human activin A using the methanolic yeast, __Pichia pastoris__. Optimization of culture procedures has involved comprehensive examination of the effects of culture vessel shape, volume of broth in the induction and expression cultures, m