Optimization of size-exclusion separation of proteins on a Superose column
β Scribed by Paul L. Dubin; Joseph M. Principi
- Book ID
- 108335862
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 410 KB
- Volume
- 479
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
On the basis of studying the retention and band broadening of proteins on the TSK SW column, diffusion coefficients (OS) of solute in stationary phase were obtained which elucidate the hydrodynamic process of chromatographic resolution of proteins by hydrophilic size-exclusion chromatography (SEC).
## Abstract The chromatographic fractionation of proteins by sizeβexclusion chromatography in a simulated moving bed (SMB) is studied. During experimental fractionation of a mixture of bovine serum albumin (BSA) and myoglobin on Sepharose Big Beads, massβtransfer effects are shown to limit the perf
A multiple (triple) detection system with one detector and recorder each was developed for the high-resolution size-exclusion chromatographic separation of proteins with a slurry packed capillary column. Fused-silica capillary columns of length 1, 2.5 and 6 m and I.D. 0.26 mm were connected in this