Optimization of resolution in micellar electrokinetic chromatography

✍ Scribed by Rasmussen, Henrik T. ;Goebel, Lisa K. ;McNair, Harold M.

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 376 KB
• Volume: 14
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240140107

✦ Synopsis

A comparison of separations conducted in sodium dodecyl sulfate (SDS) and SDS modified with Brij 35 indicates that selectivity, in Micellar Electrokinetic Chromatography (MEKC), is governed by the composition of the micellar phase. Beyond selectivity optimization, resolution may be improved by increasing efficiency or decreasing electroosmotic flow. Of these approaches, increasing capillary length [to improve efficiency) should be a more time effective means of improving separation.

where lo is the elution time of a component distributing exclusively in the mobile phase and t, , the elution time of a component distributing exclusively into the micellar phase to and t,,, may be related to vo and v, , as

where L is the length of migration It follows from eqs (3-5) that the retention time of an analyte may be expressed as