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Optimization of polymerase chain reaction-amplified conditions using the uniform design method

✍ Scribed by Yang Cao; Yuanyuan Zheng; Baishan Fang

Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
92 KB
Volume
79
Category
Article
ISSN
0268-2575

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✦ Synopsis

Abstract

Polymerase chain reaction (PCR) is a key procedure in modern molecular biology but may be adversely affected by many factors and it is difficult to optimize reaction conditions; a technically simple and effective method to set up an optimal PCR‐amplified system is required. Uniform design (UD) is a method that enables many factors to be investigated. For example, the dhaT gene from Klebsiella pneumoniae was amplified and the optimization of the amplified parameters including concentration of Mg^2+^, annealing temperature, annealing time and number of cycles based on UD was reported. After only 12 experimental trials, an optimal PCR‐amplified result was obtained, demonstrating the value of UD for optimization of PCR. Copyright Β© 2004 Society of Chemical Industry

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