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Optimization of peptide separations in reversed-phase HPLC: Isocratic versus gradient elution

✍ Scribed by C. T. Mant; T. W. Lorne Burke; R. S. Hodges


Book ID
112827024
Publisher
Springer
Year
1987
Tongue
English
Weight
877 KB
Volume
24
Category
Article
ISSN
0009-5893

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An optimization technique based on gradient elution was used to separate eleven chlorophenols by reversed phase liquid chromatography. The separation was based on gradient elution with a stepwise variation pattern of the volume fraction of organic modifier, phi, in the mobile phase. Initially, two-,