Optimization of penicillin G acylase multipoint immobilization on to glutaraldehyde–chitosan beads
✍ Scribed by Wellington S. Adriano; Edilson H. C. Filho; James A. Silva; Luciana R. B. Gonçalves
- Book ID
- 111718032
- Publisher
- Portland Press
- Year
- 2005
- Tongue
- English
- Weight
- 306 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0885-4513
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## Abstract The kinetic parameters for penicillin G hydrolysis in systems with penicillin G acylase from __Escherichia coli__ (free and immobilized on activated chitosan microbeads produced by electrostatic extrusion) were determined. The obtained kinetic results indicated that both systems (free a
## Abstract **BACKGROUND:** Penicillin G acylase (PGA) has been used extensively in the β‐lactam antibiotics industry. As a biocatalyst, it is better to use immobilized enzymes than free enzymes, therefore, the immobilization of PGA on a composite carrier consisting of an adsorbent resin and biocom