Optimization of microbial poly(3-hydroxybutyrate) recover using dispersions of sodium hypochlorite solution and chloroform
✍ Scribed by Sei Kwang Hahn; Yong Keun Chang; Beom Soo Kim; Ho Nam Chang
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 533 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Optimization was carried out for the recovery of microbiol poly(3‐hydroxybutyrate) (PHB) from Alcaligenes eutrophus. This process involved the use of a dispersion made of sodium hypochlorite solution and chloroform. The dispersion enabled us to take advantage of both differential digestion by hypochlorite and solvent extraction by chloroform. The PHB recovery (%) from cell powder was maximized using a 30% hypochlorite concentration, a 90‐min treatment time, and a 1:1 (v/v) chloroform‐to‐aqueous‐phase ratio. Under these optimal conditions, the recovery was about 91% and the purity of recovered PHB was higher than 97%. The number average molecular weight, M~n~ of recovered PHB was about 300,000 and the weight average molecular weight M~w~ was about 1,020,000, compared to the original M~n~ of 530,000 and M~w~ of 1,272,000. The moderate decrease in both M~n~ and M~w~ might be ascribed to the shielding effect of chloroform. In addition, the relatively small decrease in M~w~ probably resulted from the loss of short PHB chains which might be water soluble. The crystallinity of recovered PHB was in the range of 60 to 65%although a slightly higher crystallinity was observed when the dispersion was used. © 1994 John Wiley & Sons, Inc.