Optimization of LC–NMR. III—Increased signal-to-noise ratio through column trapping

✍ Scribed by Lee Griffiths; Rob Horton

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 224 KB
• Volume: 36
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/(sici)1097-458x(199802)36:2<104::aid-omr230>3.0.co;2-f

✦ Synopsis

SigniÐcant gains in LCÈNMR signal-to-noise ratio are obtained by selective concentration of chromatographic peaks on a chromatographic column using to Ðx the sample on the solid phase. A deuterated D 2 O organic solvent is used to remove the sample from the column and into the NMR Ñow cell, which removes the need for presaturation of solvent resonances. Deuterated solvents unrelated to the original eluent are used in order to minimize spectral overlap between solvent and solute.