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Optimization of Isopropanol and Ammonium Sulfate Precipitation Steps in the Purification of Plasmid DNA

โœ Scribed by S. S. Freitas; J. A. L. Santos; D. M. F. Prazeres

Book ID
109387407
Publisher
American Institute of Chemical Engineers
Year
2006
Tongue
English
Weight
261 KB
Volume
22
Category
Article
ISSN
8756-7938

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Quantitation of hepatitis B virus DNA in
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โœ Long T. Wen; Michael Henneberger; Nicole Nguyen; Richard A. McPherson ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 789 KB

## Abstract We developed a method to quantitate hepatitis B virus (HBV) DNA in serum by ammonium sulfate fractionation and DNA hybridization. Serum samples were precipitated with 45% saturated ammonium sulfate, resuspended in buffer, and spotted on a nylon membrane. Following denaturation in alkali