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Optimization of gene-disruption methods for reverse genetics in the nematode C. elegants

✍ Scribed by Keiko Gengyo-Ando; Shohei Mitani


Book ID
117364794
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
68 KB
Volume
31
Category
Article
ISSN
0168-0102

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## Background: Detection of transferred genes in histological sections has been problematic due to low transfection efficiency and copy number achieved with current vectors. in situ polymerase chain reaction (in situ pcr) is a new method for the detection of low-abundance nucleic acid targets in ti