Optimization of conditions for acid protease partitioning and purification in aqueous two-phase systems using response surface methodology
✍ Scribed by Draginja M. Peričin; Senka Z. Mađarev-Popović; Ljiljana M. Radulović-Popović
- Publisher
- Springer Netherlands
- Year
- 2008
- Tongue
- English
- Weight
- 272 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Correlations to describe the effect of surface hydrophobicity and charge of proteins with their partition coefficient in aqueous two‐phase systems were investigated. Polyethylene glycol (PEG) 4000/phosphate, sulfate, citrate, and dextran systems in the presence of low (0.6% w/w) and hig
## Abstract The behavior of a series of pure proteins partitioned in aqueous two‐phase systems is compared with their behavior during mild hydrophobic interaction chromatography (HIC). A simple theoretical rationale for this comparison is presented based upon solvophobic theory. Similarities were f