Optimization of chromium removal by the chromium resistant mutant of the cyanobacterium Anacystis nidulans in a continuous flow bioreactor
✍ Scribed by Jasvir IS Khattar; Tangirala A Sarma; Anuradha Sharma
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2007
- Tongue
- English
- Weight
- 144 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0268-2575
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
BACKGROUND: Chromium removal potential of the cyanobacterium Anacystis nidulans and its chromium resistant strain Cr^r^I8 has been optimized. Optimized parameters include biomass load, pH, temperature and dilution rate of the bioreactor.
RESULTS: Results show that chromium resistant strain has high EC~50~ dose for chromium compared to wild strain. Chromium removal potential of both strains is strongly influenced by various factors. Optimized conditions in batch system included pH 6.5, temperature 28 °C, biomass load 150 µg protein mL^−1^ for 30 µmol L^−1^ Cr^6+^ solution. In continuous flow bioreactor at optimum pH (6.5) and temperature (28 °C) at a fixed biomass of 10 mg protein and 30 µmol L^−1^ Cr^6+^, metal removal efficiency varied with dilution rate. For A. nidulans continuous flow bioreactor, optimum dilution rate was 0.076 h^−1^ (64.6 per cent metal removal) while for Cr^r^I8 it was 0.152 h^−1^ (85.8 per cent metal removal). Operative time of the Cr^r^I8 bioreactor was also more (85 h) compared to A. nidulans bioreactor (45 h).
CONCLUSION: Under optimized conditions resistant strain Cr^r^I8 removed more Cr^6+^ compared to A. nidulans and thus has the potential to be exploited for Cr^6+^ removal from industrial effluents at large scale. Copyright © 2007 Society of Chemical Industry