Optimization of capillary array electrophoresis single-strand conformation polymorphism analysis for routine molecular diagnostics
β Scribed by Cathrine Jespersgaard; Lars Allan Larsen; Shingo Baba; Yoji Kukita; Tomoko Tahira; Michael Christiansen; Jens Vuust; Kenshi Hayashi; Paal Skytt Andersen
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 282 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0173-0835
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We have developed a means of nonradioisotopic single strand conformation polymorphism (nonRI-SSCP) analysis and applied it to the detection of a point mutation in the human tumor suppressor gene, p53. The method does not require any particular facilities or apparatus, such as a laboratory for radioa
Capillary array electrophoresis (CAE) is a novel technique, which allows for high throughput analysis of DNA fragments. When screening for mutations in whole populations or large patient groups it is necessary to have robust and well-characterized setups for high throughput analysis. For large-scale