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Optimization of bulk cell electrofusion in vitro for production of human–mouse heterohybridoma cells

✍ Scribed by Katja Trontelj; Matej Reberšek; Maša Kandušer; Vladka Čurin Šerbec; Marjana Šprohar; Damijan Miklavčič

Book ID
108069073
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
592 KB
Volume
74
Category
Article
ISSN
1567-5394

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## Abstract This article describes the development and full characterization of a microfluidic chip for electrofusion of human peripheral blood B‐cells and mouse myeloma (NS‐1) cells to generate hybridomas. The chip consists of an array of 783 traps, with dimensions that were optimized to obtain a