Optimization of biogas yield from anaerobic digestion of silages and industrial wastes

✍ Scribed by A. Kacprzak; L. Krzystek; S. Ledakowicz

• Publisher: Elsevier
• Year: 2009
• Tongue: English
• Weight: 92 KB
• Volume: 25
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2009.06.594

✦ Synopsis

This technique is based on precision measurements of parameters of high frequency acoustical waves, propagating through the analyzed sample (reaction container). HR-US allows continuous analysis of opaque, heterogeneous or viscous media in a wide range of temperatures and pressures. It does not require sample preparation, or optical activity and can be used in opaque samples. The presented data include enzymatic hydrolysis of microcrystalline cellulose (50 mM acetate buffer solution, pH 5.0) by commercial cellulase (Celluclast 1.5 L) at 50 • C. Ultrasonic velocity and attenuation in cellulose suspensions have been measured in the frequency range from 2 to 20 MHz as a function of reaction time using a HR-US 102 ultrasonic spectrometer. Ultrasonic reaction profiles were used to analyze the reaction progress curves. Reaction rates have been compared with the measurements of concentrations of reducing sugars during the reaction using a discontinuous colorimetric assay (DNS method). Moreover, kinetic parameters and changes in particle size have been evaluated from the analysis of the ultrasonic data. Overall, these results show the high potential of HR-US for direct real time monitoring of kinetics of cellulose hydrolysis in complex systems.