Optimization of an Extraction Procedure for the Quantification of Vitamin E in Tomato and Broccoli using Response Surface Methodology
✍ Scribed by Junsoo Lee; Lin Ye; William O. Landen Jr.; Ronaldo R. Eitenmiller
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 180 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0889-1575
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✦ Synopsis
Response surface methodology (RSM) was applied to optimize the extraction procedure for the determination of vitamin E in tomato and broccoli samples. The e!ects of varying the amount of 60% potassium hydroxide (KOH), saponi"cation time, and "nal ethanol concentration (EtOH) on the tocopherol contents were evaluated to optimize extraction and saponi"cation steps by RSM. The optimized parameters were obtained by ridge analysis. Based on the ridge analysis, optimum conditions found were: ml 60% KOH, 8.4}8.9; saponi"cation time (min), 50.7}54.3 at 703C; and %EtOH, 30.1}35.0. Under the optimized conditions, the experimental values agreed with values predicted by ridge analysis. The analytical method validation parameters such as accuracy, precision, limit of detection, limit of quanti"cation, and speci"city were calculated to ensure the method's validity.