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Optimization of a Versatile in Vitro Transcription Assay for the Expression of Multiple Start Site TATA-less Promoters †

✍ Scribed by Lin, Yixin; Ince, Tan A.; Scotto, Kathleen W.

Book ID
120533224
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
182 KB
Volume
40
Category
Article
ISSN
0006-2960

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The mouse thymidylate synthase (TS) promoter is a member of a family of promoters that lack a TATA box as well as an initiator element and that initiate transcription at many sites over a broad initiation window. An element (MED-1) downstream of the initiation window of almost all promoters of this