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Optimization and validation of TaqMan Real time RT-PCR method for measurement of FOXP3, GITR and CTLA-4 gene expression

✍ Scribed by Narges Valizadeh; Abdlrahim Rezaee; Houshang Rafatpanah


Book ID
118430700
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
62 KB
Volume
44
Category
Article
ISSN
0009-9120

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Real-time RT-PCR method was exploited to identify endogenous reference genes in differentiating human T helper cells. When using this technology in our experimental system, finding a set of genes whose mRNA expression levels would not change appeared to be very challenging. Our initial plan to use t