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Optimised N-acetyl-d-lactosamine synthesis using Thermus thermophilus β-galactosidase in bio-solvents

✍ Scribed by Manuel Sandoval; Concepción Civera; José Berenguer; Francisco García-Blanco; María J. Hernaiz


Book ID
118178462
Publisher
Elsevier Science
Year
2013
Tongue
French
Weight
528 KB
Volume
69
Category
Article
ISSN
0040-4020

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## Abstract The enzymatic synthesis of N‐acetyl‐lactosamine (LacNAc) by the transgalactosylation of N‐acetyl‐D‐glucosamine (GlcNAc), catalyzed by the β‐galactosidase from Bacillus circulans (BcβGal), was studied in hydro‐organic media, starting from o‐nitrophenyl‐β‐D‐galactopyranoside (oNPG) as a g