Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor
✍ Scribed by Yan Zhou; Franky Fung-Kei Choi; Zhi Zhou He; Jing-Zheng Song; Chun-Feng Qiao; Xin Liu; Li-Sheng Ding; Suo-Lang Gesang; Hong-Xi Xu
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 217 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
A method that couples rapid, sensitive, reproducible and accurate ultra‐performance LC (UPLC) with quadrupole‐TOF‐MS was established for the first simultaneous qualitative and quantitative analysis of phenolic compounds in Artemisia minor. Box–Behnken designs (BBDs) were applied as an effective tool to optimise major parameters that influence the resolution of UPLC, including three gradient steps and column temperature. Under optimal UPLC conditions, a total of 23 phenolic compounds in the crude methanol extracts of A. minor were well separated on a Waters Acquity UPLC BEH C~18~ column (100×2.1 mm, 1.7 μm particle size) within 16.5 min, and the compounds were unequivocally or tentatively identified via comparisons with authentic standards and literature. In this study, a total of six major phenolic compounds were quantified in A. minor and the method was validated to be sensitive, precise and accurate within the LOD from 1.24 to 5.27 μg/mL, and the overall intra‐ and inter‐day variations in detection were less than 3.76%. The recovery of the method ranged from 97.9 to 103.8% with RSDs that were less than 5.8%. These results demonstrate that this approach has the potential for quality control of A. minor and other Tibetan herbal medicines.