Optimisation of the polymerase chain reaction and dot-blot hybridisation for detecting cytomegalovirus DNA in urine: comparison with detection of early antigen fluorescent foci and culture
β Scribed by P.C Evans; J Gray; T.G Wreghitt; Graeme J.M Alexander
- Book ID
- 114140599
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 371 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0166-0934
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## Abstract A biotinβlabeled DNA probe specific for the imβ mediate early gene of the cytomegalovirus (CMV) strain AD 169 was generated with the polymerase chain reaction. Nucleic acid hybridisation was carried out with CMVβinfected Tβlymphoblastoid cells (MOLT4) after 4 hr to 6 days of culture. Bi
The sensitivity of the polymerase chain reaction (PCR) assay was compared to that of standard Southern blotting (SB) hybridization for detecting the presence of Epstein-Barr virus (EBV) genomes in biopsy samples from 43 patients with a variety of lymphoproliferative disorders. Two pairs of oligonucl