Optimisation of the liquid chromatographic separation of pirimicarb and its metabolites V–VII: application to a soil sample used as a candidate reference material
✍ Scribed by M. Honing; D. Barceló; R.T. Ghijsen; U.A.Th. Brinkman
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 915 KB
- Volume
- 286
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
The simultaneous separation of the N-dimethylcarbamate pirimicarb and its metabolites V-VII was carried out by liquid chromatography (LC) using diode array detection (DAD). 'Dvo cohu~s, a 250 x 4.6 mm i.d. column packed with S-pm particles with a cyanopropyl modified silica stationary phase and a 150 X 4.6 mm i.d. column packed with 3-pm particles with a base deactivated C, modified silica stationary phase, were compared. The addition of a buffer in the eluent, for both columns, was needed to improve the peak shape of metabolite V and the resolution of the metabolites V-VII and consequently the pH dependence of the retention time was tested. Additionally chromatograms of twosoil samples of different origin, spiked at a concentration level of 1 pg g-* for pirimicarb, analysed by LC-DAD, gas chromatography (GC) with nitrogen phosphorus detection and GC-mass spectrometry are shown. Detection limits and reproducibility data of pirimicarb in a candidate reference sample using LC-DAD and a cyanopropyl column are reported. Keyworu!s: Liquid chromatography; Pirimicarb; Soils
Simultaneous analytical methods used for the isolation of polar pesticides and their degradation products are of general interest in addition to the screening methods for pesticide residue analysis in environmental matrices. This is evident from the number of publications dealing with the analysis of degradation products of various pesticides like carbaryl[1,2], carbofuran [3-51, aldicarb [6-81 and atrazine [9,10] in environmental matrices.