Optimisation of protein expression and establishment of the Wave Bioreactor for Baculovirus/insect cell culture
โ Scribed by Wilfried Weber; Eric Weber; Sabine Geisse; Klaus Memmert
- Book ID
- 110403625
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 285 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0920-9069
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The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. Sf9 cells in SF90011 medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of lactate and ammonia. Tn5 cells in Excell 401 medi
Per cell protein expression in virally-infected insect cells declines significantly at high cell density resulting in a decrease in volumetric productivity. Specific protein expression levels in Spodoptera frugiperda (Sf-21) cells could be increased at high cell densities by increasing the oxygen su